In order to investigate the resistance of Salmonella in four animals(cattle, chicken, pig and sheep) to the commonly used antibacterial drugs in clinic in Yanqi, Xinjiang and the status of carrying resistance genes, selective medium and PCR detection of housekeeping gene invA were used to isolate and identify Salmonella. Agar dilution method was used to detect the minimum inhibitory concentration of 11 antibacterial drugs and PCR method was used to detect 14 drug-resistant genes in the isolates. The results showed that 191 strains of Salmonella from different animal sources were isolated, and the isolation rates from high to low were cattle(62.0%), chicken(28.3%), pig(9.0%) and sheep(8.5%). The isolated Salmonella from different animals showed different levels of resistance to the tested antibacterial drugs. The severity of the drug resistance of chickens, sheep, pigs and cattles decreased in order. The drug resistance spectrum of Salmonella from chickens were wide, the multidrug resistance were distributed in 0-3, 5-7, and the resistance rates to tetracycline and florfenicol were above 70.0%. The resistance rate of Salmonella from sheep to ampicillin(88.2%) was higher than those of other animal sources, and the main multidrug resistance was 7-resistant(23.5%). The resistance rate of Salmonella from cattle to ciprofloxacin(66.1%) was higher than those of other animal sources, and the main multidrug resistance was 4-resistant(38.7%). Salmonella from different animal sources were sensitive to fosfomycin, imipenem and polymyxin, and the resistance spectrum were diversified. Except qnrS and mcr-1 genes were not detected in Salmonella from different animal sources, the other drug-resistant genes were detected. The blaCMY-2 and tetA genes were only detected in Salmonella from pigs, different animal-derived Salmonella mainly carried blaTEM, blaOXA, tetB, oqxA, oqxB, aadA2, ant(3")-Ia, aac(6')-Ib-cr and floR drug resistance genes at the same time. The detection rates of resistance genes were the highest among Salmonella from cattles, above 35%, about 20% among Salmonella from sheep and pigs and below 10% among Salmonella from chickens.