大雅柑枯斑病斑的病原鉴定及防治药剂的筛选
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科技部国家重点研发计划项目(2017YF D0202006–04);农业部国家现代农业柑橘产业技术体系(CARS–26–05B)


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    摘要:

    2020年春季,于重庆市长寿区柑橘园内采集大雅柑典型枯斑病果实,采用组织块分离法分离得到菌株CS11,对其进行形态学和分子生物学鉴定,确定CS11为Stagonosporopsis cucurbitacearum。向健康的柑橘盆钵苗喷洒CS11孢子悬液可致植株患病,从患病样品中分离得到菌株CS11,确认CS11是致病病原菌;CS11对大雅柑(Citrus reticulata.cv. dayagan)、有核沃柑(Citrus rtemple× Dancy)、塔罗科血橙(Tarocco blood orange(Citrus sinensis(L.) Osbeck))和尤力克柠檬(Citrus limon)的叶、枝、果实均具有致病性。室内药剂筛选结果显示,75%肟菌?戊唑醇水分散粒剂、60%唑醚?代森联可湿性粉剂、450 g/L咪鲜胺乳油、37%苯醚甲环唑可湿性粉剂、11.5%吡唑嘧菌酯乳油5种杀菌剂对CS11有较好的抑制作用,其中 75%肟菌?戊唑醇水分散粒剂、60%唑醚?代森联可湿性粉剂、37%苯醚甲环唑可湿性粉剂、11.5%吡唑嘧菌酯乳油等4种药剂的EC50值均小于10 mg/kg。

    Abstract:

    In the spring of 2020, the typical diseased citrus fruits were collected in Changshou, Chongqing, and the blight fruits of dayagan(Citrus reticulate cv. dayagan) were obtained. The strain CS11 was obtained from the diseased samples by tissue separation. Through morphology and molecular biology characterization, strain CS11 was determined as Stagonosporopsis cucurbitacearum(S. ucurbitacearum). Pathogenicity study by spraying the spores on health citrus plants showed that strain CS11 caused blight on citrus, which confirmed the pathogenicity of strain CS11. S. ucurbitacearum strain CS11 has pathogenicity on Citrus reticulate cv. Dayagan, C. rtemple×Dancy, C. sinensis (L.) Osbeck (Tarocoo blood orange) and C. limon. And the strain CS11 is pathogenic to the leaves, branches and fruits of the 4 kinds of citrus. The growth rate method was used to determine the virulence of different agents to the strain, the results showed five fungicides including 75% trifloxystrobin tebuconazole, 60% pyraclostrobin metiram, 450 g/L prochloraz, 37% difenoconazole and 11.5% pyraclostrobin had good inhibitory effect on strain CS11. The EC50 values of 75% trifloxystrobin tebuconazole, 60% pyraclostrobin metiram, 37% difenoconazole and 11.5% pyraclostrobin were all less than 10 mg/kg.

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占爽,杨静,胡军华,吴玉珠,吴望,张嘉,陈娜,周彦.大雅柑枯斑病斑的病原鉴定及防治药剂的筛选[J].湖南农业大学学报:自然科学版,2021,47(5):.

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  • 在线发布日期: 2021-11-02
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