Tea seeds were used as raw materials, which were vacuumed and freeze-dried, then crushed and manually sprayed with sterile water to create tea seeds powder with 10%, 15% and 20% water content. Toxin producing Aspergillus flavus (ACCC30899) was artificially inoculated on the tea seeds powder which was cultured in biochemical incubator and observed continuously to investigate the growth of Aspergillus flavus. Aflatoxin components were measured in tea seeds whose water content were more than 15% during the infection of Aspergillus flavus (7 d, 14 d and 21 d) by liquid chromatography tandem mass spectrometry. It turns out that Aspergillus flavus could grow in tea seeds with more than 10% water. When the water content was more than 15%, Aspergillus flavus grew well and the aflatoxin contents were all lower than the detection lower limit (0.03 μg/kg), indicating tea seeds with water content higher than 15% have a small risk of producing aflatoxin under the condition of toxic Aspergillus flavus(ACCC30899) infection.