赵以桥,余烨颖,吕雅婷,曾昱龙,,胥伟.黄曲霉侵染茶树籽产黄曲霉毒素的研究[J].湖南农业大学学报:自然科学版,2021,47(4):.
黄曲霉侵染茶树籽产黄曲霉毒素的研究
  
DOI:
中文关键词:  茶树籽  含水量  黄曲霉  黄曲霉毒素
英文关键词:tea seeds  water content  Aspergillus flavus  aflatoxins
基金项目:科技部国家重点研发计划项目(2019YFC0840503);四川省科学技术厅重点研发计划项目(2019YFN0178)
作者单位
赵以桥,余烨颖,吕雅婷,曾昱龙,,胥伟 1.四川农业大学园艺学院四川 成都 6111302.精制川茶四川省重点实验室四川 成都 611130 
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中文摘要:
      以茶树籽为原料,真空冷冻干燥后粉碎并喷无菌水,使含水量分别为10%、15%、20%,人工接种产毒黄曲霉(Aspergillus flavus )ACCC30899,置于生化培养箱,观察产毒黄曲霉的生长状况;采用液相色谱串联质谱法分别于黄曲霉侵染7、14、21 d、含水量大于15%的茶树籽所产黄曲霉毒素组分进行测定。结果表明:茶树籽粉末含水量高于10%时黄曲霉可生长;茶树籽粉末含水量大于15%时黄曲霉生长状态良好,黄曲霉毒素组分测定值均低于检测下限(0.03 μg/kg),提示在产毒黄曲霉侵染含水量大于15%的茶树籽,产黄曲霉毒素的风险较小。
英文摘要:
      Tea seeds were used as raw materials, which were vacuumed and freeze-dried, then crushed and manually sprayed with sterile water to create tea seeds powder with 10%, 15% and 20% water content. Toxin producing Aspergillus flavus (ACCC30899) was artificially inoculated on the tea seeds powder which was cultured in biochemical incubator and observed continuously to investigate the growth of Aspergillus flavus. Aflatoxin components were measured in tea seeds whose water content were more than 15% during the infection of Aspergillus flavus (7 d, 14 d and 21 d) by liquid chromatography tandem mass spectrometry. It turns out that Aspergillus flavus could grow in tea seeds with more than 10% water. When the water content was more than 15%, Aspergillus flavus grew well and the aflatoxin contents were all lower than the detection lower limit (0.03 μg/kg), indicating tea seeds with water content higher than 15% have a small risk of producing aflatoxin under the condition of toxic Aspergillus flavus(ACCC30899) infection.
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