王春波,吕辉,韦玲冬,郭治友?.贵定雪芽茶树高EGCG品系代谢组学的比较研究[J].湖南农业大学学报:自然科学版,2021,47(4):.
贵定雪芽茶树高EGCG品系代谢组学的比较研究
  
DOI:
中文关键词:  贵定雪芽茶  代谢组学  超高效液相色谱–四级杆串联飞行时间质谱
英文关键词:Guidingxueya tea  metabolomics  ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS)
基金项目:国家自然科学基金项目(31660056);贵州省教育厅青年科技人才成长项目(黔教合KY字[2020]211)
作者单位
王春波,吕辉,韦玲冬,郭治友? 黔南民族师范学院生物科学与农学院贵州 都匀 558000 
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中文摘要:
      基于超高效液相色谱–四级杆串联飞行时间质谱(UPLC–QTOF–MS)分析,运用XCMS软件、METLIN数据库和SIMCA–P软件,对贵定雪芽茶树表没食子儿茶素没食子酸酯(EGCG)含量较高的NW34品系和对照组品系NW48进行了代谢物定性分析、定量分析和差异性筛选等代谢组学的比较研究,共鉴定出26种显著差异代谢物,包括氨基酸及多肽类(8种)、黄酮类(4种)、有机酸类(4种)、核酸及其衍生物类(3种)、生物碱类(2种)、醛类(2种)、糖类(1种)、酚类(1种)和烯类(1种)。通路富集分析显示这些差异代谢物主要分布于12个代谢途径中,其中类黄酮类生物合成代谢途径包含的差异代谢物最多(5种),芳香类化合物降解途径次之(4种)。NW34品系含有的EGCG、表儿茶素(EC)、糖类和氨基酸含量高于NW48品系,而咖啡碱含量却低于NW48品系,表明贵定雪芽高EGCG茶树NW34更适合加工成绿茶。
英文摘要:
      Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS) was used to analyze the metabolites between Guidingxueya tea(NW34) containing higher epigallocatechin 7-O-gallate (EGCG) and the control group(NW48). XCMS software, METLIN database and SIMCA-P software were used to perform qualitative analysis, quantitative analysis and differential screening of metabolites. A total of 26 significantly different metabolites were identified, including 8 amino acids and peptides, 4 flavonoids, 4 organic acids, 3 nucleic acids and their derivatives, 2 alkaloids, 2 aldehydes, 1 sugar, 1 phenol, and 1 alkene. Pathway enrichment analysis showed that these differential metabolites are mainly distributed in 12 metabolic pathways, of which flavonoid biosynthesis contains the most differential metabolites(5 metabolites), followed by the degradation of aromatic compounds pathway(4 metabolites). In addition, the EGCG, epicatechin(EC), carbohydrates and amino acids metabolites contained in the NW34 strain are significantly higher than those in the NW48 strain, while the content of caffeine is lower than that in the NW48 strain, which indicates that the NW34 tea plant is more suitable for processing into green tea.
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