In order to isolate high quality genomic DNA from tea plant leaves enriching polyphenols and polysaccharides, this paper is an attempt to compare the DNA extracting effect of four modified methods including CTAB general method, CTAB subarea method,SDS general method and SDS subarea method.Besides the CTAB subarea method is used for the first time for genomic DNA isolation from tea plant.Results show that the value of OD 260 / OD 280 for all the DNA samples obtained with these four modified methods is over 1.8, the molecular weight of DNAs is more than 21 kb, and DNAs are completely digestible with restriction endonucleases and amplifiable in the polymerase chain reaction (PCR), indicating freedom from common contaminating compounds. The high quality genomic DNA can be obtained with any method if the process is reasonable.