不同光强对辣椒卷叶突变体D26卷曲指数及光合特性的影响
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湖南省研究生科研创新项目(CX20240642);云南省重大科技专项(202402AE090012)


Effects of different light intensities on the curling index and photosynthetic characteristics of the pepper leaf-curling mutant D26
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    摘要:

    以辣椒A124为对照,以卷叶突变体D26为试验材料,设置弱光((25±5) μmol/(m2?s))、中光((200±5) μmol/(m2?s))和强光((500±5) μmol/(m2?s))3种光照强度处理,连续处理15 d后,测定叶片卷曲指数、生物量、叶绿素含量、气体交换参数及叶绿素荧光参数,并结合石蜡切片观察叶片细胞结构变化,系统分析卷叶突变体D26在不同光强下的生长特性及光合生理特征。结果显示:光照强度对辣椒叶片形态影响显著;D26在弱光下叶片平展,在中光、强光下叶片卷曲(叶片卷曲指数分别为19.63%和43.89%);2个材料在中光、强光、弱光条件下的总生物量均依次降低;相比A124,D26在弱光下的生物量积累能力较强,对弱光适应力更佳;中光影响D26叶片卷曲但不抑制生物量积累,强光则使D26叶片生物量显著降低;强光下D26叶片细胞形态和排列结构发生改变,进而引发叶片卷曲;弱光下叶片变薄,组织结构疏松;不同光强处理下,D26叶绿素含量高于A124的,其在弱光下通过积累叶绿素增强光捕获能力,中光下叶绿素含量降幅较大,强光下显著降低;弱光下,D26和A124的光合速率无明显差异,中、强光下D26的气孔导度变小,蒸腾速率降低,导致光合速率下降;D26在弱光下可维持良好的光合效能,中、强光下PSⅡ反应中心活性较低,但可通过维持较高的非光化学猝灭系数(NPQ)启动光保护机制,从而适应强光环境胁迫。

    Abstract:

    Using normal-leaf pepper A124 as the control and the leaf-curling mutant D26 as the test material, three light intensity treatments were applied: low light ((25±5) μmol/(m2?s)), moderate light ((200±5) μmol/(m2?s)), and high light ((500±5) μmol/(m2?s)). After 15 days of continuous treatment, leaf-curling index, biomass, chlorophyll content, gas exchange parameters, and chlorophyll fluorescence parameters were measured. In addition, paraffin sectioning was employed to observe changes in leaf cellular structure. The growth characteristics and photosynthetic physiological traits of the leaf-curling mutant D26 under different light intensities were systematically analyzed. The results showed that light intensity significantly affected pepper leaf morphology. Under low light, D26 leaves remained flat, whereas under medium and high light intensities, the leaves rolled up, with leaf-curling indices of 19.63% and 43.89%, respectively. For both accessions, total biomass decreased sequentially under medium, high, and low light conditions. Compared with A124, D26 had stronger biomass accumulation capacity and better adaptability under low light. Medium light induced leaf curling in D26 but did not affect its biomass accumulation, while high light significantly reduced leaf biomass in D26. Under high light, changes in the morphology and arrangement of leaf cells in D26 led to leaf curling. Under low light, the leaves became thinner and had a looser structure. Under different light intensities, the chlorophyll content of D26 was higher than that of A124. D26 enhanced light capture by accumulating chlorophyll under low light, showed a large decrease in chlorophyll content under medium light, and exhibited significantly reduced chlorophyll content under high light. Under low light, the photosynthetic rates of D26 and A124 were similar. Under medium and high light, stomatal conductance and transpiration rate of D26 decreased, leading to a reduction in photosynthetic rate. D26 maintained good photosynthetic efficiency under low light. Although the activity of its PSⅡ reaction center was lower under medium and high light, D26 could initiate photoprotection through a higher NPQ level to adapt to high light stress.

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黄宇,单虹虹,周鸿宇,欧立军*.不同光强对辣椒卷叶突变体D26卷曲指数及光合特性的影响[J].湖南农业大学学报(自然科学版),2026,52(2):36-44.

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  • 在线发布日期: 2026-07-13
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