辣椒SEP亚家族基因鉴定及CaSEP3表达分析
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特色蔬菜产业技术体系项目(CARS-24-A-15)


Identification of SEP subfamily and expression analysis of CaSEP3 in pepper
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    摘要:

    利用ST-8樟树港辣椒全基因组鉴定得到6个SEPALLATA(SEP)亚家族蛋白编码基因,对其理化性质、染色体定位、编码蛋白保守基序和保守结构域进行分析;将辣椒SEP基因与拟南芥、番茄、水稻和玉米的SEP基因共同构建系统进化树;探究CaSEP3的组织表达水平及编码蛋白的亚细胞定位。结果表明:辣椒SEP基因分布于5条染色体上,编码蛋白都含有MADS结构域(MADS_MEF2_like)和K结构域(K-box);辣椒SEP基因可分为2类,即SEP3和SEP1/2/4,这与番茄和拟南芥SEP基因的进化树类似。辣椒CaSEP3是拟南芥AtSEP3的同源蛋白,定位于细胞核中,CaSEP3在花蕾、胎座和果皮中高表达,推测其参与辣椒花器官和肉质果实的发育。常见激素和非生物胁迫处理后(0~24 h),脱落酸(ABA)、H2O2、NaCl可使CaSEP3表达量上调,其余处理下CaSEP3在叶片和根部的表达量变化不明显。

    Abstract:

    Six coding genes of SEPALLATA(SEP) subfamily members were identified through genome-wide analysis of the ST-8 Zhangshugang pepper. The physicochemical properties, chromosome localization, encoded protein conserved motifs and conserved structures of the subfamily members were analyzed. The phylogenetic tree was constructed with the SEP genes of pepper, Arabidopsis, tomato, rice and maize. Furthermore, tissue expression level of CaSEP3 and subcellular localization of encoded protein were explored. The results showed that SEP genes of pepper were distributed on five chromosomes, with all their encoded protein contained MADS domain(MADS_MEF2_like) and K-box domain. SEP genes could be divided into two branches: SEP3 and SEP1/2/4, which was similar to the evolutionary development of tomato and Arabidopsis. CaSEP3 was a homologous protein of AtSEP3, which was localized in the nucleus. CaSEP3 was highly expressed in flower buds, placenta and pericarp, which was speculated to be involved in development of flower and fleshy fruit in pepper. After common hormone and abiotic stress treatments(0~24 h), the expression of CaSEP3 was up-regulated by ABA, H2O2 and NaCl, while the expression of CaSEP3 in leaves and roots did not change significantly under other treatments.

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梁心仪,汤冰倩,张新昊,邹学校,刘峰 *.辣椒SEP亚家族基因鉴定及CaSEP3表达分析[J].湖南农业大学学报(自然科学版),2025,51(4):15-24.

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  • 在线发布日期: 2025-09-29
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