Using the flue-cured tobacco cultivar ‘Yunyan 87’ as the plant material, techniques including enlarging the aperture of the seedling tray, amending the seedling substrate with bacterial fertilizer, delayed transplanting of long-leaf-age seedlings cultivated under sparse sowing conditions, and incorporating microbial inoculants into the base fertilizer were integrated to promote early growth and rapid establishment. The effects of these different accelerated establishment modes on agronomic traits, dry matter accumulation and distribution, nutrient accumulation and distribution, and fertilizer use efficiency were investigated. The results indicate that T1 treatment(136 well seedling tray+seedling substrate amended with “Miaoqiangzhuang” bacterial fertilizer+ “Genjangkang” microbial inoculant, transplanted at 10-leaf stage), T2 treatment(200 well seedling tray+seedling substrate amended with “Miaoqiangzhuang” bacterial fertilizer+ “Genjangkang” microbial inoculant, transplanted at 8-leaf stage) both promoted aboveground growth of tobacco, and enhanced the accumulation of dry matter, nitrogen, phosphorus, and potassium in the plants. Compared with traditional planting methods, the T1 treatment resulted in higher efficiency metrics. The dry matter accumulation efficiencies of N, P and K fertilizers increased by 31.10%, 31.09% and 31.07%, respectively. Corresponding increases in tobacco leaf production efficiencies were 33.28%, 33.28% and 33.19%, while absorption efficiencies rose by 34.08%, 40.22% and 14.79%, respectively. The T2 treatment also showed improvements: dry matter accumulation efficiencies increased by 24.59%, 24.58% and 24.59%; leaf production efficiencies by 20.25%, 20.31% and 20.22%; and absorption efficiencies by 22.76%, 38.78% and 12.18%. In the tobacco-rice double cropping area, a strategy involving large-cell seedling trays, bacterial fertilizer amendment in seedling substrate to raise seedlings suitable for delayed transplanting, and microbial inoculants in base fertilizer promoted early and rapid tobacco establishment, showing considerable promise for widespread extension