Based on the genome and transcriptome information of Siraitia grosvenorii, the TCP transcription factor family SgTCP6 gene was screened and analyzed by bioinformatics, and whether it was involved in the regulation of the key enzyme gene SgUGT94 in the synthesis of mogroside was verified by yeast one-hybrid. The result showed that SgTCP6 gene CDS was 618 bp in length, encoded 205 amino acid residues, and had a relative molecular mass of 21?942. It was subcellularly localized in the nucleus and was an unstable hydrophilic protein without a signal peptide or transmembrane region, thus making it a non-secretory protein. Multiple sequence alignment results showed that the gene was closely related to TCP in Momordica charantia. The co-expression analysis results of genes in Siraitia grosvenorii fruits at different stages showed that there was a significant correlation between the expression of SgTCP6 and SgUGT94. The analysis results of the 2 000 bp promoter upstream of SgUGT94 revealed that this region contained four TCP binding sites. The yeast one-hybrid results showed that SgTCP6 might be involved in the regulation of the key enzyme gene SgUGT94 in the synthesis of mogroside V.