In this study, CsSCL23(the homologous protein of SCL23) in cucumber was obtained through phylogenetic tree of SCL23. The CsSCL23 gene was cloned from cucumber inbred line 649, and its molecular characteristics and expression characteristics were studied. The results showed that there was only one CsSCL23 protein in cucumber, which was most closely related to melon CmSCL23 protein. The protein contains a conserved GRAS motif sequence and is a typical GRAS family gene. The CDS of CsSCL23 gene was 1 335 bp in length, encoding 444 amino acids. The relative molecular mass of the protein was 48 766 230, the theoretical isoelectric point was 6.04, the instability coefficient was 56.93, the fat coefficient was 91.17, and the total average hydrophilicity was –0.245, with no signal peptide and transmembrane domain, and belonged to unstable protein. The secondary and tertiary structure prediction results showed that the cucumber CsSCL23 protein was composed of four structures, including 45.27% α-helix, 4.27% β-turn, 10.14 % extended strand and 40.32% random coil. The cis-acting element prediction results of CsSCL23 promoter showed that there were gibberellin and abscisic acid binding related elements in this region. Gene expression pattern analysis results showed that CsSCL23 was highly expressed in flowers, roots and petioles of cucumber. After exogenous hormones gibberellin and abscisic acid were applied to cucumber roots, CsSCL23 was significantly up-regulated in the elongation zone and mature zone of cucumber roots in response to the stimulation of gibberellin and abscisic acid. After cucumber seedlings were treated with 100 μmol/L exogenous auxin and abscisic acid, it was found that the expression of CsSCL23 gene was significantly up-regulated at 6 h after treatment, indicating that CsSCL23 gene may mediate the regulation of plant hormones on the growth and development of cucumber plants.