At the ages of 1, 60, 120, 180, 240, 300, and 360 days(recorded as ND1, ND60, ND120, ND180, ND240, ND300, and ND360 respectively), the longissimus dorsi muscle of 3 Ningxiang pigs were respectively selected for transcriptome sequencing analysis. An enrichment analysis was performed on the differentially expressed genes(DEGs) screened from 9 comparison groups. The levels of DEGs were verified using real-time fluorescence quantitative polymerase chain reaction(qRT-PCR). The results showed that 1 982, 245, 131, 311, 26, 84, 2 257, 1 377, and 2 654 DEGs were identified in ND60 vs(short for versus) ND1, ND120 vs ND60, ND180 vs ND120, ND240 vs ND180, ND300 vs ND240, ND360 vs ND300, ND180 vs ND1, ND360 vs ND180, and ND360 vs ND1 comparison groups, respectively. Gene ontology(GO) analysis indicated that the DEGs were mainly involved in muscle contraction, skeletal muscle tissue development, and calcium ion binding processes. Kyoto encyclopedia of genes and genomes(KEGG) pathway analysis showed that the DEGs were mainly enriched in signaling pathways such as gluconeogenesis/glycolysis, PI3K-Akt, and MAPK. The qRT-PCR verification of the DEGs showed the expression trends were consistent with the sequencing results. Through transcriptome sequencing and experimental verification, we found that THBS3 was downregulated in the ND180 vs ND1 and ND360 vs ND1 comparison group, and THBS4 was significantly downregulated in ND180 vs ND1 and upregulated in ND360 vs ND180, indicating the expression patterns of THBS3 and THBS4 were opposite to the growth and development pattern of pigs. It is speculated that those may negatively regulate the growth and development of skeletal muscles.