Using flue-cured tobacco variety‘K326’as material, the effects of vertical deep tillage(DT), vertical deep tillage plus decomposed organic fertilizer(DTY), vertical deep tillage plus humic acid(DTF) and vertical deep tillage plus biological bacterial fertilizer(DTJ) on soil properties, dry matter accumulation of flue-cured tobacco, chemical composition coordination, physical properties, sensory quality and appearance quality of tobacco were comprehensively analyzed. The results showed that compared with DT treatment, the contents of soil available potassium, organic matter, total nitrogen, available phosphorus and dry matter accumulation of tobacco plants were significantly increased under DTY and DTF treatment. DTY treatment also improved the chemical component availability index(CCUI), total appearance quality score(AQC) and flue-cured tobacco economic benefit index(ECI) of the inverted 2 and 4 tobacco leaves. The total sensory quality score(SQC), physical property index(PPI) and economic character index(ECI) of the inverted 2 and 4 tobacco leaves were improved by DTF treatment. On the whole, vertical deep tillage combined with different exogenous substances could improve the soil fertility and adjust the quality of the upper tobacco leaves to a certain extent, in which vertical deep tillage combined with mature organic fertilizer had a better effect on the chemical composition coordination and appearance quality of the inverted 4 and 6 leaves of the upper tobacco leaves, while vertical deep tillage combined with humic acid could improve the physical characteristics and sensory quality of the inverted 2 and 4 leaves of flue-cured tobacco.