To investigate the protective effect of raspberry crude polysaccharides(RCP) against ultraviolet B(UVB)-induced skin photodamage in BALB/c mice, RCP was administered to UVB-induced BALB/c mice skin photodamage model at doses of 50, 100 and 200 mg/kg and the levels of matrix metalloproteinases, inflammatory factors and oxidative stress-related indices in skin tissue were measured by enzyme-linked immunosorbent assay(ELISA) or microplate assay. The results showed RCP significantly alleviated erythema, desquamation, wrinkles, local hyperkeratosis, epidermal thickening, fibrous tissue hyperplasia and lymphocytic infiltrate of the skin damaged by UVB. Compared to the model group, in the low-dose group, there was a 16.1% reduction in tumor necrosis factor-α(TNF-α) levels, accompanied by a decrease of 22.1% and 43.3% in matrix metalloproteinase-1(MMP-1) and matrix metalloproteinase-3(MMP-3) content, respectively. In the medium-dose group, malondialdehyde(MDA) content decreased by 18.9%, TNF-α levels dropped by 37.4%, and MMP-1 and MMP-3 content decreased by 35% and 48.2%, respectively. For the high-dose group, superoxide dismutase (SOD) and catalase (CAT) activities were enhanced by 21.3% and 34.3%, respectively, while MDA content decreased by 24.8%. Interleukin-6(IL-6) and TNF-α levels were reduced by 33.3% and 56.6%, respectively. Additionally, MMP-1 and MMP-3 content decreased by 35% and 63.3%, respectively. The above results indicated that RCP could ameliorate UVB-induced skin photodamage by inhibiting matrix metalloproteinases content, attenuating inflammation response and oxidative stress in BALB/c mice, and the high dose of RCP showed the best effect.