湖南省猪轮状病毒分子流行病学调查及遗传变异分析
DOI:
CSTR:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

湖南省自然科学基金联合基金项目(2023JJ5005)


Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    2023年1—12月从湖南省9个地区119个规模化猪场采集1 036份仔猪腹泻粪便样品,采用RT–PCR检测猪轮状病毒(PoRV)的流行率,并对PoRV的VP7基因进行扩增并测序;采用MegAlign 软件进行序列同源性分析,通过MEGA 7.0 软件构建系统进化树。结果表明:仔猪腹泻样品检出PoRV阳性样本168份,阳性率约为16.22%;冬季PoRV的检出率高于春、夏、秋季的;从PoRV阳性样本中扩增出9个VP7基因序列,G9为优势基因型(占比约77.8%),其次为G4型和G5型,二者占比均为11.1%。综上,湖南省腹泻仔猪粪便中PoRV的流行率高且基因型复杂,其中G9型为优势基因型。

    Abstract:

    From January to December 2023, the 1 036 samples were collected from 119 pig farms in 9 representative regions of Hunan Province. The prevalence of Porcine rotavirus(PoRV) were detected by RT-PCR, and then positive VP7 amplification samples was sequenced. Sequence homology was analyzed by MegAlign software. Phylogenetic tree was constructed by using MEGA 7.0 software. Of the 1 036 samples examined, 16.22% were positive for PoRV. The prevalence of PoRV in winter was significantly higher than those in other three seasons. Among the 9 sequenced VP7 sequences, G9(77.8%) was the dominant genotype, followed by G4 and G5, both accounting for 11.1% of the total genotypes. These results indicated that the prevalence of PoRV in pig farms was high and the genotypes were complex in Hunan Province. The G9 was the dominant genotype in pig farm in Hunan Province.

    参考文献
    相似文献
    引证文献
引用本文

梁跃,肖犟,尹杰,黄颖,伍玉梅,苏焕,禹爱春,莫凌云,刘国华*.湖南省猪轮状病毒分子流行病学调查及遗传变异分析[J].湖南农业大学学报:自然科学版,2024,50(6):.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2025-01-17
  • 出版日期:
文章二维码