In order to prolong the room temperature shelf-life of pomegranates, the electron beam generated X-ray (EBGX) was applied with 0.5, 1.0, 1.5 kGy doses of soft seed pomegranate ‘Tunisi’ with control group(CK) of no dose treatment, and the experimental shelf-life was 20 d. The quality indexes were collected every 5 d. The quality indexes of each treatment group during the room temperature shelf-life were compared to obtain the optimal EBGX dose. The results showed that during the room temperature shelf-life, the EBGX had no significant influence on the change of soluble solid content, and the 0.5 kGy EBGX could significantly inhibit the pH increase. The treated group with 0.5 kGy EBGX showed the smallest increase in the relative conductivity and the total peel color difference ?E*of pomegranates, and the lowest respiratory intensity at the end of the trial. And, the titratable acid content decreased slowly in the 0.5 kGy EBGX treatment group, and its browning degree was significantly lower than those of the other groups. During the same period, the pomegranates in the 0.5 kGy EBGX treatment group received the highest sensory evaluation scores. In summary, the 0.5 kGy EBGX dose showed the optimal treatment in this study and this treatment condition is recommend to be used for the storage and preservation of pomegranate to prolong the shelf-life at room temperature.