To accelerate the development of functional genomic research, the mutant library of ‘Hongyang’ kiwifruit was constructed. The callus of ‘Hongyang’ kiwifruit were treated with 60Co-γ rays at 10 distinct radiation doses: 10, 25, 50, 75, 100, 150, 200, 300, 400, and 500 Gy, with 0 Gy serving as the control, and the phenotypic variation characteristics of the mutagenic progeny were analyzed. The results indicated that after mutagenic treatment with a dose of 100 Gy, the mutation rate of callus tissue from ‘Hongyang’ kiwifruit increased to 35.9%, while the differentiation rate reached 77.8%. Based on these findings, a radiation dose of 100 Gy was selected as the further studied condition．After treating 1 680 ‘Hongyang’ kiwifruit callus under optimal mutagenesis conditions, a kiwifruit mutant library contained 1 026 regenerated seedlings of ‘Hongyang’ kiwifruit was constructed. The mutant library had rich variation in leaf, plant type and other characters, with a total variation frequency of 35.9%.