This study employed genomic resequencing technology to analyze the genotype and the genetic structure of Xiangjia Black Chicken, Xiangjia White Broiler, Xiangjia Yellow Chicken No.2, Xiangjia Black Chicken No.2 and Shaobo Chicken populations, and to identify high-quality single nucleotide polymorphism(SNP) loci. Based on the variance components between 90 day old body weight(BW90) and 70 to 90 day old residual feed intake(RFI) and the breeding value estimations, it was found that the accuracy of breeding value estimations using the “Jingxin No.1” 10K SNP loci subset was above 0.915. Functional locus annotation using the QTLdb database led to the selection of 2 851 significant SNP loci associated with economic traits of broilers. Ultimately, 60 784 high-quality and highly polymorphic SNP loci were identified for the design of the “Xiangxin No.1” broiler 60K liquid phase breeding chip. The detection results of the chip show that the genotype consistency of the “Xiangxin No.1” broiler 60K liquid phase breeding chip is around 95%, and both individual and locus detection rates are maintained above 99%, indicating that the breeding chip has good genomic prediction effects.