Characteristics of 3 types of biochar(shallow carbonized rice husk biochar, deep carbonized rice husk biochar, and tobacco stem biochar) were studied, and a field experiment was conducted using tobacco variety Xiangyan7 as the material. Treatment groups received equal amounts(1 t/hm2) of shallow carbonized rice husk biochar, deep carbonized rice husk biochar, and stalk biochar, respectively, and the control group received no biochar. The physical and chemical properties of the soil, agronomic traits of the tobacco plants, and the physical and chemical properties of the tobacco leaves after flue curing were measured. The results showed that the deep-carbonized rice husk biochar exhibited the maximum number of surface pore and the biggest surface area. Compared to the control group, the pH of the soil in tobacco stem biochar treatment was raised by 14.37%, and the available nitrogen in the shallow carbonized rice husk biochar treatment was increased by 20.31%. The plant height, stem diameter, and maximum leaf area of the tobacco plants in the shallow carbonized rice husk biochar treatment were 34.92%, 18.60%, and 24.92% higher than those in the control group, respectively. Compared to the control group, the nitrogen accumulation in the shallow carbonized rice husk biochar, tobacco stem biochar, and deep carbonized rice husk biochar treatments was increased by 56.7%, 26.27%, and 41.49%, respectively. Stems of cured upper tobacco leaves in the shallow carbonized rice husk biochar treatment was increased by 22.91%, cracked leaves in lower leaves was increased by 17.97%, and the chemical components were within the range of high-quality tobacco leaves. The application of shallow carbonized rice husk biochar had better effects on improving soil physical and chemical properties, on nutrient accumulation in tobacco plants, and on the growth and quality of flue-cured tobacco.