Goat rumen epithelial cells (GRECs) were cultured in medium containing 1 μg/mL of LPS for 3 h, 6 h and 9 h to detect cell viability, antioxidant indices, genes expression of inflammatory factors and tight junction protein. The results showed as follows: 1) The viability of GRECs in the group treated with LPS for 3 h was significantly lower than that in groups treated with LPS for 6 h and 9 h, but there was no significant difference in GRECs activity between groups treated with LPS for 6 h and 9 h. 2) The glutathione peroxidase (GSH-PX) activity of GRECs treated with LPS for 3 h was extremely significantly lower than that in cells treated with LPS for 6 h and 9 h (P<0.01), whereas the malondialdehyde(MDA) content, on the contrary, was extremely significantly higher in cells treated with LPS for 3 h. The superoxide dismutase (SOD) and catalase (CAT) activities of GRECs significantly increased and the reactive oxygen (ROS) concentration extremely decreased with the prolongation of LPS treatment time. 3) The relative expression levels of TNF-ɑ and IL-1β in GRECs treated with LPS for 3 h were significantly higher than that in cells treated with LPS for 6 h and 9 h. The relative expression of IL-1β was significantly decreased with the extension of LPS treatment time. The relative expression levels of IL-6 had no significant difference among all group. 4) The distribution of ZO-1 was low in GRECs treated with LPS for 3 h, but increased with the extension of LPS treatment time. The relative expression levels of Claudin-1 in GRECs treated with LPS for 3 h were significantly higher than that in cells treated with LPS for 6 h and 9 h. The results showed that the GRECs could alleviate oxidative damage by improving their own antioxidant and anti-inflammatory capacities with the extension of LPS treatment time, then improve barrier function of the cells.