The fungal pathogen was obtained from the leaves of Echeveria cv. ‘Peach Pride’ with suspected rot disease based on tissue separation and single spore separation. According to morphological observation, molecular identification and pathogenicity test, this pathogen was identified as Fusarium oxysporum(F. oxysporum). The main pathogenic substances of F. oxysporum was determined, it was found that the pathogen has strong degrading enzyme activity on cell wall and the ability to produce toxins(fusaric acid), indicating that cell wall degrading enzymes and toxins are the main factors for the pathogen’s invading, colonizing and pathogenesis.