番茄红素微胶囊的制备及抗炎活性分析
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国家自然科学基金面上项目(22178070);内蒙古自治区科学技术厅科技兴蒙项目(2021CG0029)


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    摘要:

    以辛烯基琥珀酸淀粉钠和麦芽糊精分别加阿拉伯胶为壁材,制备番茄红素微胶囊,测定番茄红素微胶囊的包封率,分析其在不同温度条件下的稳定性;采用体外脂多糖(LPS)诱导RAW264.7细胞、BPH–1细胞与体内佛波酯(TPA)诱导小鼠耳肿胀模型,探讨番茄红素微胶囊的抗炎活性及其抗炎机制。结果表明:辛烯基琥珀酸淀粉钠和阿拉伯胶质量比为2∶1的复合壁材制备得到的番茄红素微胶囊的包封率为85.43%,在45 ℃条件下,番茄红素微胶囊中番茄红素保留率超过50%;在0.5~10.0 μg/mL范围内,番茄红素微胶囊对LPS诱导分泌NO的抑制率为8.83%~72.33%,其IC50为3.76 μg/mL;10.0 μg/mL番茄红素微胶囊对BPH–1细胞增殖的抑制率高达52.17%,其IC50为8.33 μg/mL;番茄红素微胶囊对TPA诱导小鼠耳肿胀的抑制率为38.45%,显著抑制TNF–α、IL–6、MDA、ROS细胞因子过度表达,抗炎作用优于番茄红素油树脂。可见,番茄红素微胶囊比番茄红素油树脂具有更好的稳定性及抗炎活性,其作用机制与抑制TNF–α、IL–6、MDA、ROS过度表达有关。

    Abstract:

    To investigate the stability and anti-inflammatory activity of lycopene microcapsules in vitro and in vivo, in this study, lycopene microcapsules were prepared by using starch sodium octenylsuccinate and maltodextrin with arabic gum respectively as wall materials. The encapsulation efficiency, storage stability of lycopene microcapsules under different temperature conditions were evaluated. The anti-inflammatory activity and mechanism of lycopene microcapsules were evaluated by using LPS-induced RAW264.7 cells, BPH-1 cells in vitro and TPA induced ear swelling mice model in vivo. The encapsulation rate obtained by using starch sodium octenylsuccinate+arabic gum at the a mass ratio of 2∶1 was 85.43%, and the retention rate of lycopene was more than 50% at 45 ℃. In the range of 0.5-10.0 μg/mL, the inhibition rate of lycopene microcapsules on LPS-induced NO secretion ranged from 8.83% to 72.33%, with an IC50 of 3.76 μg/mL; and the inhibition rate of 10.0 μg/mL lycopene microcapsules on the proliferation of BPH-1 cells was as high as 52.17%, with IC50 8.33 μg/mL. Lycopene microcapsules inhibited TPA-induced ear swelling in mice by 38.45%, and also significantly inhibited the overexpression of TNF-α, IL-6, MDA, and ROS cytokines, and the anti-inflammatory effect was superior to that of lycopene oleoresin. In summary, lycopene microcapsules had improved stability and anti-inflammatory activity over lycopene oleoresin, and their mechanism of action was linking to the inhibition of the overexpression of TNF-α, IL-6, MDA, and ROS.

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李文治,马诗经,何文江,郭高辉,王珊珊,杜志云.番茄红素微胶囊的制备及抗炎活性分析[J].湖南农业大学学报:自然科学版,2024,50(1):.

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  • 在线发布日期: 2024-03-22
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