To identify the species of ticks in cattle and to investigate the Anaplasma spp. in ticks, 572 ticks were collected from 7 provinces and regions (Henan, Hunan, Hainan, Sichuan, Guizhou, Guangdong and Guangxi) of China. Morphological and molecular biological methods were used to identify the species of these ticks, and PCR was conducted to analyze Anaplasma infection in these ticks. Sequence analysis showed that 572 ticks were identified as Rhipicephalus microplus(R. microplus), and three clades(A, B and C) of R. microplus were found. the sequence of 16S rRNA gene of Anaplasmas amplified from ticks showed R. microplus carried the Anaplasmas. The prevalence of Anaplasmas in ticks was 38.8%(222/572), and the prevalence of Anaplasmas marginale, Anaplasmas capra, Anaplasmas platys and Anaplasmas phagocytophilum were 23.6%(135/572), 7.3%(15/572), 5.3%(42/572), 2.6%(30/572), respectively. Co-infection of Anaplasma species was only found in ticks from Hunan Province. This is the first report of R. microplus clade C in China, and Anaplasma margina is the common Anaplasma species.