Diseased leaves from Zingiber mioga with leaf blight were collected from severely affected fields, and pathogenic bacteria were isolated and purified using tissue isolation method. The representative pathogenic strain RH-1 was obtained, further identification of strain RH-1 was conducted and biological characteristics of the strain were determined. Combining the results of morphological characteristics, molecular and biological identification, constructed fungal internal transcriptional spacer regions(ITS), phylogenetic tree based on β-Tubulin and TEF-1α, the pathogen was identified as Fusarium oxysporum. Strain RH-1 was cultured on PDA medium with different temperatures(5, 10, 15, 20, 25, 30, 35 ℃), pHs(4, 5, 6, 7, 8, 9, 10), and light durations(continuous light, continuous darkness, 12 hours of light/12 hours of darkness, and 24 hours of light/24 hours of darkness). The results showed that the mycelium grew faster under 30 ℃, pH9, and continuous light conditions. Using glucose α-lactose and maltose as carbon sources, and yeast extract, beef extract, (NH4)2SO4, NH4Cl, peptone, and NH2CH2COOH as nitrogen sources to replace the carbon and nitrogen sources in Czapek basic culture medium in equal amounts. mycelium growth showed that the optimal carbon source was glucose or maltose, and the optimal nitrogen source was beef extract or peptone. Strain RH-1 was inoculated into red and green Zingiber mioga respectively, and it was found that green Zingiber mioga has stronger disease resistance.