A total of 180 juvenile hybrid sturgeon with body mass of (32.81±1.28) g were randomly divided into 4 groups and fed Ampelopsis grossedentata powder supplemented with 0.00%(A0), 0.50%(A1), 1.00%(A2) and 2.00%(A3) diets for 60 days with 3 replicates in each group and 15 fish in each replicate. The growth performance, serum biochemical and immune indexes, intestinal morphology indexes, digestive enzyme activity and microbial diversity, muscle component content and amino acid composition of hybrid sturgeon were collected respectively. The results showed that compared with A0, the final mass, mass gain rate, specific growth rate and survival rate of A1 and A2 hybrid sturgeon had no significant changes, while the final mass, mass gain rate and specific growth rate of A3 were significantly decreased. The contents of total cholesterol, triglyceride, albumin and activity of glutamic oxaloacetic transaminase(AST) of A1 were significantly increased, while the AST activities of A2 and A3 were significantly decreased. Serum alkaline phosphatase, catalase, glutathione peroxidase and superoxide dismutase activities, total antioxidant capacity and intestinal protease and amylase activities were significantly increased in experimental groups, while the lipase activity of A2 was significantly increased and that of A1 was significantly decreased. The intestinal fold height of A1 and A2 were significantly increased. The species diversity of intestinal flora in A2 was increased. There were no significant changes in muscle routine components in all experimental groups. The ratio of essential amino acids to total amino acids in A2 and A3 muscles were significantly increased. In conclusion, under the conditions of this study, appropriate addition of A. grossedentata powder could improve the immune level of hybrid sturgeon without affecting its growth indexes, and the optimal addition amount is 1.00%.