The previously identified full-length transcripts of Apis mellifera ligustica were aligned to the Nr database by Blast tool, a total of 49 genes and their 550 full-length transcripts relevant to the Hippo signaling pathway were identified. The identified 550 full-length transcripts relative to the Hippo signaling pathway were compared with the annotated transcripts in the Apis mellifera (A. mellifera) reference genome (Amel_HAv3.1) using the gffcompare software, the 5' UTRs of 9 genes and the 3' UTRs of 7 genes annotated to the Hippo signaling pathway in the A. mellifera reference genome were respectively prolonged. Based on the Astalavista software, 7 alternative splicing (AS) events in 4 genes were identified, including two exon skipping, two intron retention and three alternative 5' splicing sites. The authenticity of AS events in 1 gene was verified by PCR. On basis of the TAPIS pipeline, 24 genes associated with the Hippo signaling pathway were identified to contain at least 1 alternative polyadenylation (APA) sites individually; among them, the genes containing 1 APA site were the most abundant. By using the TBtool software, the consensus sequence of HVNCCNBNDYVDVVHVNDBVDYCNBBDNNNHNNVNNVMNN was identified.