In this study, the iron-oxidizing bacterium EEELCW01, isolated from As-contaminated soils, underwent comprehensive genome analysis. The potential function of As-related genes was assessed through comparative analysis with GO, KEGG and COG databases, while the As transformation capacity of this strain was investigated via hydroponic experiments. Results unveiled that EEELCW01 possessed a genome size of 4 714 242 bp, encompassing two chromosomes sized at 2 065 078 bp and 2 649 164 bp, respectively, with a GC content of 55.99%. The chromosomes harbored 4588 CDSs, 58 tRNAs and 12 rRNAs. COG annotation emphasized gene functions centered on amino acid transport and metabolism, as well as inorganic ion transport and metabolism. GO annotation highlighted functions such as integral membrane components, oxidation-reduction processes and related enzyme activities. KEGG annotation predominantly indicated metabolism-related genes. The strain’s genome featured multiple As metabolism-related genes, including arsC, arsH, arsB, arsR, acr3, arrA, arxA and arsM. Hydroponic experiments exhibited the strain’s robust capability to reduce As(Ⅴ) to As(Ⅲ), manifesting a reduction rate of 40.1% after 3 days of incubation. In conclusion, through promoting As bioreduction and being in combination with hyperaccumulator, the EEELCW01 could be expected to conduct for the remediation of As-contaminated environments.