Flue-cured tobacco laminae (Fujian Nanping C3F, Yunnan Qujing C3F, Guizhou Qianxinan C3F and Guizhou Qianxinan C4F) aged for 3 years were used as test materials, the contents of conventional chemical components including total alkaloids, total sugars, reducing sugars and total nitrogen, advanced fatty acids including linoleic acid, oleic acid, linolenic acid, hexadecanoic acid and octadecanoic acid and polyphenolic compounds including chlorogenic acid, cryptochlorogenic acid, scopolamine and rutin during excessive aging were analyzed through continuous sampling and detection for 5 years. The result showed that when exceed the appropriate aging period, the contents of total sugar, reducing sugar, linolenic acid, chlorogenic acid, cryptochlorogenic acid and rutin in tobacco laminae gradually decreased with the advance of storage stagnation. According to the principal component analysis, the comprehensive scores of different samples decreased year by year during the stagnation period, and significant quality loss of the flue-cured tobacco occurred after 2-3 years of storage delay. Comparison between C3F and C4F grade flue-cured tobacco laminae from Qianxinan showed the comprehensive score of C3F grade after 4 years of storage detention was lower than that of C4F grade before storage detention. It could be seen that too long storage detention would lead to the degradation of tobacco quality. Comparison among C3F grade flue-cured tobacco laminae from Nanping, Qujing and Qianxinan regions showed that Qujing tobacco leaves were relatively resistant to storage, and Nanping tobacco leaves were not resistant to storage.