In this study, a 1, 10-phenanthroline spectrophotometry for the determination of phytic acid content in rice was established. The protocol included three steps, first add excess Fe3+ and phytic acid to form 4∶1 PA(Fe)4 precipitation, then use hydroxylamine hydrochloride to reduce the remaining Fe3+ to Fe2+, after that use 1,10-phenanthroline as a color developer to quantitatively detect Fe2+ by spectrophotometry, and finish the detection of phytic acid content in rice. The pH and reaction time of the determination system was optimized, and analyze the effect of inorganic phosphoric acid on the determination of phytic acid content, the reproducibility and accuracy of the method were verified. The results showed that the phytic acid concentration could be accurately determined in the range of 6.7×10-7-2.5×10-5 mol/L under the pH 2.5 and reaction time was 30 min, and the R2 of the working curves for the determination of phytic acid at low and high concentrations were greater than 0.99. The free inorganic phosphate did not affect the detection of phytic acid when the inorganic phosphate concentration was lower than 2.0×10-5 mol/L. The recoveries were 93.30%-104.67% and the RSD of 1.25%-7.34% for five parallel tests on standard samples of 6.7×10-7-2.5×10-5 mol/L. The phytic acid contents of the rice of four varieties, including Huanghuazhan, Jinsuo408, Kitaake and Nipponbare, determined by this method were 5.42, 4.72, 6.40, 5.68 mg/g, the RSD were 1.23% to 7.60%.