The pathogen was isolated and purified from diseased leaves collected from 4 tea-producing bases and identified as Pseudopestalotiopsis theae by morphological and molecular biological identification plus pathogencity test. The identified pathogen was used as indicators to screen the antagonistic strain from diseased leaves by plate confrontation method. Strain kc-6 with the strongest antagonistic effect was identified as Bacillus amyloliquefaciens and the antifungal rate was 79.86%. The antagonistic ability of strain kc-6 was further investigated by analyzing pathogen mycelial growth, in vitro inhibitory effect on the pathogen, subculture stability of strain kc-6 and the antagonistic effect on 10 pathogens. The result showed that strain kc-6 can cause the pathogenic fungal mycelium to shrivel up and become thin and partially expanded; the relative inhibition rate of original fermentation broth, the 10 times diluted fermentation broth and the 100 times diluted fermentation broth of strain kc-6 to Pseudopestalotiopsis theae were 72.73%, 52.55% and 3.03% respectively; the inhibition rate of strain kc-6 remained at 70.33% after subcultured 25 times; strain kc-6 could inhibit 10 tested pathogens and the inhibition rate against Phomopsis vexans, Phytophthora melonis and Fusarium graminearum were 83.00%, 80.00 % and 76.00 %, respectively.