In order to evaluate the salt tolerance of Amaranthus cruentus germplasms from different geographical sources, compare their differences in salt tolerance, and breed new salt-tolerant A. cruentus varieties, 18 A. cruentus germplasm resources were germinated under 250 mmol/L NaCl solution and sterilized distilled water in this study. The indicators of germination and growth, such as relative germination potential, relative germination rate, relative root length, relative bud length, relative germination index and relative vigor index were collected in the experiments, and the membership function and cluster analysis were used to evaluate the salt tolerance. The results indicated that bud and root lengths of all tested materials were inhibited with more the inhibition in roots. The inhibition on seed germination and seedling growth under salt stress showed significant diffence. The membership function and clustering results showed the studied 18 A. cruentus germplasm resources could be divided into three groups: 8 germplasms with strong salt tolerance, Ace613, Ace660, Ace568, Ace655, Ace641, Ace659, Ace643 and Ace649, 7 germplasms with moderate salt tolerance, Ace647, Ace621, Ace656, Ace671, Ace631, Ace62 and Ace650 and 3 germplasms with weak salt tolerance, Ace651, Ace617 and Ace575.