In this study, the open reading frame(ORF) sequence of GIH-like gene of Macrobrachium rosenbergii (MrGIH-like) was obtained by using RT-PCR, the expression of MrGIH-like in different tissues was detected by qPCR, and prokaryotic technology was used for the recombinant protein of MrGIH-like expression. The results showed that the ORF of MrGIH-like was 339 bp in length, which encoded 112 amino acid residues contain 34 amino acid signal peptide and 78 amino acid comprising the mature peptide, and MrGIH-like gene possessed a typical structure motif from the CHH-II family. The analysis of homology and phylogenetic tree showed that MrGIH-like had the highest identity and the shortest evolutional distance with GIH of Macrobrachium nipponense. Quantitative Real-Time PCR analysis showed that the expression of MrGIH-like was the highest in eyestalk and very low in brain, nerve neuro, heart and other tissues, and the expression level was significantly(P<0.01) different between eyestalk and the other tissues. The relative molecular mass of MrGIH-like recombinant protein was about 2.6×104 and about 8.5×103 after removed the tag fusion protein, which was very close to the relative molecular mass(9.4×103) predicted by Prot Param.