Abstract:The supervised field trials of trifloxystrobin in apples was carried out in the 12 main apple producing areas in China, including Qingdao of Shandong, Yinchuan of Ningxia and Dalian of Liaoning, etc. The residues of trifloxystrobin and its metabolite CGA321113(trifloxystrobin acid) in the collected apple samples were extracted with acetonitrile, fixed volume with ethyl acetate after suction filtration, salting out and concentration, and then detected by gas chromatography. The dietary risk of trifloxystrobin was evaluated. The results showed at the additional levels of 0.05, 0.50 and 5.00 mg/kg, the average recoveries for trifloxystrobin and CGA321113 in the apples were 89%-99% and 89%-98% with relative standard deviation were 6%-10% and 7%, respectively. The limit of quantitation both were 0.05 mg/kg. The trifloxystrobin was sprayed on apples for 3 times with an effective component of 100 mg/kg, and the spraying interval was 7 d. At 28th day after the last pesticide application, the residue of trifloxystrobin in apples were <0.05-0.14 mg/kg, the residue of CGA321113 were <0.05-0.06 mg/kg, and the total residue of trifloxystrobin in apple were <0.10-0.19 mg/kg. On the 21st day, the residue of trifloxystrobin in apples were <0.05-0.15 mg/kg, the residue of CGA321113 were <0.05-0.28 mg/kg, and the total residue of trifloxystrobin in apple were <0.10-0.37 mg/kg. The maximum total residue was lower than the maximum residue limit of trifloxystrobin in apple formulated by China. The national estimated daily intake of trifloxystrobin was 0.19 mg and the risk quotient was 7.5%. The risk assessment result showed that the dietary intake risk of trifloxystrobin was at an acceptable level.