To explore the effects of three types of organic fertilizers on the yield, quality and content of medium and trace elements of spring tea, two-year field trials were performed in the ‘Longjing43’mature tea garden in the hilly area of western Zhejiang. Four fertilization treatments were set including chemical alone fertilizer as control and three organic fertilizers, which were made by pig manure, tea residue, and biogas residue, and organic fertilizer replaces 50% of chemical fertilizer. The results showed that compared with chemical fertilizers, after continuous application of organic fertilizer for 2 a, the organic fertilizers from pig manure, tea residue, and biogas residue increased the yield of fresh leaves of spring tea by 21.53%, 3.98% and 6.62%, respectively. Compared with chemical fertilizers, the total amount of free amino acids in tea treated with organic fertilizers from tea residues and biogas residues increased after 1 a and 2 a of application. The phenol-ammonia ratio of the organic fertilizer from tea residues was significantly reduced after 1 a of application. The phenol-ammonia ratios of the three kinds of organic fertilizers significantly reduced after 2 a of application, and the improvement effect of organic fertilizer application for 2 a on tea quality was more obvious than that for 1 a. The data of tea yield and quality in the two consecutive years were comprehensively evaluated using the principal component analysis, and the comprehensive scores of tea residue, pig manure, biogas residue and only chemical fertilizer were decreased in turn. The content of Cu and Zn in tea leaves treated with pig manure organic fertilizer were the highest, and the content of Ca, Mg, Fe, and Mn in tea leaves treated with tea residue organic fertilizer were the highest. It could be seen that the application of the three organic fertilizers could increase the yield of tea, improve the quality of tea, and promote the absorption of medium and trace elements in tea, especially the application of tea residues organic fertilizer in tea gardens was better than the other two fertilizer.