In this study, the silage was mixed with Myriophyllum elatinoides and wheat bran at the fresh mass ratio of 7∶3 with addition of certain amount of Lactobacillus plantarum and 0%, 2%, 4% and 6% glucose, respectively. The fermentation quality, nutritional quality and aerobic stability of the mixed silage were measured to evaluate the effect of the glucose on the quality of mixed silage. The results showed the addition of glucose could significantly(P<0.05) reduce pH, butyric acid, NH3-N/TN, crude protein and crude fiber mass fraction of mixed silage, and increase lactic acid mass fraction, further optimized fermentation characteristics. Adding 4% glucose significantly reduced mass fraction of neutral detergent and acid detergent fiber(P<0.05). The aerobic stability of the mixed silage treated with 0%, 2%, 4% and 6% glucose was >360, 226, >360 and 62 h, respectively. The comprehensive evaluation results showed that with the combined condition, the fresh mass ration of 7∶3, with 2% glucose addition, the mixed silage of Myriophyllum elatinoides and wheat bran could achieve the improved fermentation quality, nutritional quality and aerobic stability.