The host range of rice root-knot nematodes(Meloidogyne graminicola) in Hunan rice region were systematically identified by field investigation and inoculating 27 kinds of crops with second stage juveniles (J2) of nematode in greenhouse conditions from 2020 to 2021. The results showed that these nematodes can infect 29 species of plants from 15 families, including 15 weeds, 12 vegetable crops and 2 grain crops. Zea mays, Dioscorea esculenta, Arachis hypogaea, Capsicum annuum, Lycopersicon esculentum, Cucurbita moschata, Benincasa hispida, Cucumis melo L. var. Flexuosus Naud., Luffa cylindrica, Cucurbita pepo, Abelmoschus esculentus and Canavalia gladiata were non-host, while Glycine max, Triticum aestivum, Phaseolus vulgaris, Daucus carota, Allium fistulosum, Allium sativum and Brassica rapa var. chinensis were poor hosts. Non-host and poor host crops could be applied in crop rotation measures.