The resistance to Plasmodiophora brassicae(P. brassicae) of 100 rapeseed varieties cultivated in Hunan Province in recent years was evaluated by artificial inoculation in greenhouse and natural induction. Results showed that 57 cultivars were resistant to P. brassicae when estimated by artificially inoculation in greenhouse. Among the 100 cultivars in Yuanling and Hengyang, there were 45 and 8 resistant cultivars in the two regions, respectively. Combined the results of greenhouse and field resistance identification, 7 resistant rapeseed cultivars were identified, including Liangjing811, Zhongyanyou1, Huaxin402, Fengyou737, Baoyou150, Youyan9 and Jiayou1427.