To investigate the petal senescence-related gene PoERF113 of ‘Fengdan’ (Paeonia ostii) expression patterns in petals, different tissues and different varieties at different flowering stages and their response to ethylene, we did cloning analyzed its coded protein properties in this study. First, we used RT-PCR technology to clone the petal senescence related gene PoERF113 gene from ‘Fengdan’. The gene belongs to AP2/ERF family. Its open reading frame(ORF) is 636 bp, encoding a protein consisting of 211 amino acid residues. It contains one AP2 conservative domain and does not contain transmembrane structure. The molecular weight of the protein is about 53 000, and the theoretical isoelectric point (pI) is 5.13. It is unstable protein and hydrophilic. The results of qRT-PCR analysis showed that PoERF113 gene was expressed in the open color stage, the first open stage, the half open stage, the full bloom stage, the first decline stage, and the decline stage, mainly in the petals and leaves of ‘Fengdan’ in the open color stage; The highest expression was found in ‘Fengdan’ mutant strain of Zaohua; The sequence of PoERF113 gene in the ‘Fengdan’ mutant line of Zaohua was the same as that in ‘Fengdan’ and there was no base difference; PoERF113 gene was responsive to ethylene, and its expression level gradually increased with the extension of treatment time and growth process. It was speculated that this gene might be related to the process of flower senescence induced by ethylene. This experiment laid a preliminary foundation for further study on the function of PoERF113 gene and its molecular mechanism in peony petal senescence and florescence regulation.