In order to screen out nitrogen-efficient wheat cultivars, 34 wheat varieties were used as raw materials by use of principal component analysis and cluster analysis to comprehensively evaluate nitrogen utilization efficiency. The field experiment was carried out in Zaoyang City, Hubei Province, from November of 2018 to May of 2019 and from November of 2019 to May of 2020. The results showed that there were differences in nitrogen efficiency indicators and yield among different wheat cultivars, and the coefficient of variation was 12.40%-37.32%. The first two principal components of the two growth cycles were extracted by principal component analysis, and the comprehensive score of nitrogen efficiency was calculated. In the first studied period, the rotation eigenvalues of the first principal component and the second principal component were 3.376 and 2.068, cumulatively reflects 90.73% of the information of the original variable. In the second studied period, the rotation eigenvalues of the first principal component and the second principal component were 4.290 and 1.327, which accumulatively reflect 93.61% of the information of the original variable. The comprehensive score of nitrogen efficiency of wheat was positively correlated with yield, and the higher the comprehensive score of nitrogen efficiency, the higher the yield. With K-Means cluster analysis, we could pick out the seven varieties with stable nitrogen efficient: Anong 0711, Chuanmai 104, E-mai 18, E-mai 006, E-mai 580, Fumai 1228 and Huamai 1168.