In order to reduce the occurrence of root rot of Panax notoginseng(P. notoginseng), the water extracts of 8 traditional Chinese medicines(Curcuma longa, Artemisia capillaris, Lonicerae japonicae, Portulaca oleracea, Scutellaria baicalensis, Viola yedoensis, Coptis chinensis, Phellodendron chinense) were used to inhibit Fusarium oxysporum(F. oxysporum)，one of the pathogens of root rot of P. notoginseng. The minimum inhibitory concentration(MIC) of 8 traditional Chinese medicines against F. oxysporum was determined by double dilution method, and the antifungal activity of water extracts of Chinese medicines against F. oxysporum was determined by growth rate method. The results showed that the water extracts of 8 kinds of traditional Chinese medicines had inhibitory effects on F. oxysporum. Among them, the water extract of L. japonicae has the best fungicidal effect on F. oxysporum, the maximum fungicidal rate was 75% and the EC50 was the lowest being 5.55 mg/mL. Coptis chinensis, Lonicerae japonicae and Scutellaria baicalensis all showed MIC≤1 mg/mL against F. oxysporum, indicating their effective initial antifungal concentration against F. oxysporum was low.