50 healthy Landrace×Large White pigs were randomly divided into 5 groups. The groups Ⅰ, Ⅱ, Ⅲ, Ⅳ were supplemented respectively with 30, 150, 300, 600 mg of Boluohui Pulvis per kilogram of feed, which were equivalent to 1, 5, 10, 20 times of the recommended clinical dose. While the control group(CK) was treated without Boluohui Pulvis. All groups were then fed for 60 days continuously and collected the effects of different doses of Boluohui Pulvis on growth performance, hematological indexes, serum biochemical indexes and histopathology of pigs. The potential mechanism of Macleaya cordata extract(MCE) against colitis was explored based on network pharmacology at the same time. The results showed that 30 mg/kg of Boluohui Pulvis could significantly increase the average daily gain and reduce the feed conversion rate of the experimental pigs, and no significant effect on growth performance in other groups pigs were observed. The hematological and serum biochemical indexes of the experimental pigs in the 30-600 mg/kg group were not significantly changed, and no abnormal pathological damage was found in the organs and tissues of pigs. Therefore, pigs fed with 30-600 mg/kg Boluohui Pulvis for 60 days had good tolerance. With SwissTargetPrediction, Genecards, STRING and other tools and databases, it was revealed that MCE might play roles using ESR1, BCL2L1, MTOR, MCL1, PTGS2, MAPK8, MMP9, MAPK1, ERBB2 and PIK3CA and other signaling pathways such as ErbB and TNF exert anti-inflammatory effects.