Yeast supported iron nanoparticles(TB-Fe/SC NPs) was prepared by using yeast as carrier and waste tobacco leaf extract as reducing agent. The removal of 2, 4, 6-trichlorophenol(2, 4, 6-TCP) by the as-prepared TB-Fe/SC NPs in aqueous solutions was investigated. The results indicated that the iron nanoparticles loaded on the yeast was spherical with a particle size of (77.6±16.1) nm. The removal of 2, 4, 6-TCP by TB-Fe/SC NPs mainly consisted of two pathways of adsorption and oxidative degradation, and the 2, 4, 6-TCP removal well follows the pseudo-second order kinetics. Dissolved oxygen and hydroxyl radicals played important roles in the oxidative degradation of 2, 4, 6-TCP. The removal efficiency of 2, 4, 6-TCP increased with the increase of temperature, and decreased with the increase of the initial concentration of 2, 4, 6-TCP, while the solution pH influences the 2, 4, 6-TCP removal slightly. The removal efficiency of 2, 4, 6-TCP increased with the increase of TB-Fe/SC NPs at the dosage of 1.0 g/L to 3.0 g/L. Fe3+, Mg2+, Ca2+ and SO42– exhibits an obvious promoting effects on the removal of 2, 4, 6-TCP by TB-Fe/SC NPs, HPO42–, HCO3- and humic acid inhibits the removal of 2, 4, 6-TCP significantly, while K+, NO3– and Cl– have no obvious influence on the removal of 2, 4, 6-TCP. This TB-Fe/SC NPs could be stored stably at room temperature for more than 6 months. The removal efficiency of 2, 4, 6-TCP decreased from 80.54% to 28.75% after six repeated applications. The removal efficiency and removal amount of 20 mg/L 2, 4, 6-TCP by 3 g/L TB-Fe/SC NPs were (80.54±0.39)%, (5.23±0.03) mg/g, respectively, at 25 ℃ and pH=5.0.