Pathogen separation from tissue was carried out on the diseased part of green crisp plums with blight collected in Wanzhou, Chongqing. Pathogen strains HYL2-2 and HYL2-5 were obtained, through traditional morphological identification and molecular biology(ITS and TUB2) identification methods, the pathogens were identified as Neopetalotiopsis sp. and Pestalotiopsis microspore. Pathogenicity test carried out on healthy green crisp plums showed the two pathogenic bacteria are pathogenic to the branches, leaves and fruits of the green crisp plums. Indoor drug screening using mycelial growth rate method showed among 16 fungicides, 25% pyraclostrobin SC has obvious antibacterial effects on Neopetalotiopsis sp., with EC50 of 0.055 4 μg/mL; 60% pyraclostrobin metiram WG has obvious antibacterial effects on Pestalotiopsis microspore, with EC50 of 0.002 μg/mL.