To identify whether the RR family member OsSSRP(salt-sensitive RR Protein, LOC_Os08g35670) of rice was response to salt stress, using the nipponbare as the material, we employed bioinformatics analysis including gene blast, domain prediction and promoter analysis of their sequences. It showed that OsSSRP contained only REC domain motif, and its promoter elements possessed large number of abscisic and jasmonic acid responsive elements. Induced expression profile analysis found that OsSSRP responded to salt and drought stress and induction of cytokinin, abscisic acid, and jasmonic acid. After seedling stage identification under 0, 40, 100 mmol/L NaCl and seed germination stage identification under 150 mmol/L NaCl, it was found that the homozygous mutant ssrp targeted knockout using CRISPR / Cas9 technology was more sensitive to salt stress. The mutant survival rate of salt stress was much lower than that of the wild type. OsSSRP was closely related to abiotic stress and hormone response, providing potential gene resources for abiotic stress resistance breeding in the rice.