In order to understand the difference of rape uptake and utilization on different selenium sources, we studied the growth, Se uptake and utilization of rape and the form of residual selenium in soil among different sources of Se (control, selenium ore, selenium powder, selenate, selenite) based on pot experiment. The results showed that different sources selenium had no significant changes in the biomass of each part of rapeseed at the mature stage. Compared with the control, the application of selenium ore, selenate and selenite significantly increased the selenium concentration in each part of rape. The selenium accumulation curve of rape was close to the “S” shape. After applying selenium ore, the maximum accumulation rate of selenium in rapeseed appeared in the flowering stage, and when other forms of selenium fertilizers were applied, the maximum accumulation rate of selenium in rapeseed appeared in the budding stage. After applying selenate, the selenium content of each part of rapeseed was significantly higher than that of other treatments, and the selenium concentration of rapeseed seed was as high as 44.56 mg/kg, the selenium concentration of selenate, selenite, selenium ore, selenium powder, and control treated rapeseed decreased in turn. After the application of selenium ore, the proportion of selenium transferred to rape seeds was the highest, reaching 23.20%. After the rapeseed was harvested, the contents of selenate, selenite, selenium ore, selenium powder, and the control treatments in soil residual available selenium decreased in turn. In conclusion, selenate has the strongest effect on selenium enrichment in rapeseed, followed by selenite, and selenium ore could also significantly promote selenium enrichment in rapeseeds, Selenium powder has no significant selenium enrichment effect.