In this study, we isolated and identified the pathogen of a unknown leaf spot disease occurred on Pachysandra terminalis (P. terminalis) plants, in May 2019 in Changsha, Hunan Province of China. Based on morphological characteristics, pathogenicity test, and molecular identification which was conducted by constructing the phylogenetic tree using combined gene sequences of internal transcribed spacer (ITS) rDNA, actin (ACT), chitin synthase 1 (CHS1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Based on the above results, we can conclude that the leaf spot disease of P. terminalis was caused by Colletotrichum liriopes.