水稻抗白叶枯病基因全基因组关联分析
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湖南省农业科技创新资金项目(2021CX35)


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    摘要:

    对水稻多样性群体(RDP–I)中的216份种质接种白叶枯生理小种P2并进行抗性鉴定,发现温带粳稻亚群平均抗性水平最高,其平均病斑长度最短;奥斯稻亚群平均抗性水平最低,平均病斑长度最长。通过全基因组关联作图鉴定了分布在水稻第1、2、4、6、7、8、9、10、11、12号染色体上的59个QTL,这些位点包含5个已知的抗白叶枯病基因。从较高阈值SNP位点以及附近2 Mb区段进行候选基因的预测,筛选出40个抗白叶枯病相关基因,并最终鉴定出16个抗性较好的水稻种质资源。

    Abstract:

    Resistance of rice diversity panel 1(RDP-I) including 216 varieties against bacterial blight was identified by inoculating the varieties with Xoo P2. Temperate japonica rice showed the highest average resistant level, with the shortest average length of disease spot; aus rice subpopulation showed the lowest average resistance, with the longest average length of disease spot. Genome-wide association study(GWAS) was used to analysis the resistance against the XooP2 of the Rice Diversity Panel 1(RDP-I). Disease evaluation of the RDP-I indicated that the TEJ subpopulation conferred a higher level of resistance to P2 than other subpopulations. Genome-wide association study(GWAS) showed that 59 QTLs were identified that distributing on chromosomes1, 2, 4, 6, 7, 8, 9, 10, 11 and 12 respectively, five cloned rice bacterial blightresistance genes were also located in the regions. From the higher p value SNP site and the 2 Mb segment near the site, we performed the prediction and screened 40 anti-bacterial blight associated candidate genes, and 16 rice varieties with high resistance against bacterial blight were identified.

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刘茁,彭莎莎,陈建芝,周亚林,李成刚,王丹.水稻抗白叶枯病基因全基因组关联分析[J].湖南农业大学学报:自然科学版,2022,48(1):.

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  • 在线发布日期: 2022-03-02
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