Pot culture was used to treat 10 lettuce varieties(‘Yidali’, ‘Hainanqingcuiboli’, ‘Sijinaichoutai’, ‘Naire’, ‘Dasu’, ‘Zhongguotaiwanxiuqiu’, ‘Ribenjieqiu’, ‘Ribenziye’, ‘Hongxiaziye’, ‘Faguozihonghuaye’) under Cd stress (CdCl2 2, 10 mg/kg), and the cadmium content of leaves and roots of each plant was measured. The Cd tolerance of lettuce was comprehensively evaluated based on the stress tolerance coefficients of plant height, leaf width, root length, shoot dry mass, root dry mass and chlorophyll content of lettuce seedlings. The cadmium tolerance coefficient of lettuce growth traits was transformed into three independent comprehensive indexes by principal component analysis, including 86.22% of all data. Through the membership function method, the comprehensive evaluation value D of Cd tolerance of lettuce at seedling stage was calculated, and the lettuce varieties were clustered. The 10 lettuce varieties were divided into four types: Cd tolerance(‘Faguozihonghuaye’, ‘Hongxiaziye’, ‘Sijinaichoutai’), moderate Cd tolerance(‘Ribenziye’), high Cd tolerance(‘Yidali’, ‘Naire’) and Cd sensitivity(‘Ribenjieqiu’, ‘Hainanqingcuiboli’, ‘Dasu’, ‘Zhongguotaiwanxiuqiu’). The mathematical model of Cd tolerance evaluation of lettuce was constructed by stepwise regression method, which was results showed that leaf width and plant height had significant effects on Cd tolerance of lettuce, which can be used as identification indexes for Cd tolerance evaluation of lettuce. Yidali and Naire plants have low cadmium enrichment, belonging to Cd tolerant varieties with low Cd accumulation in edible parts.