Upper leaves from flue-cured tobacco cultivar K326 were harvested at one time after maturity. The upper leaves were from position 1, position 2, position 3, position 4, position 5 and position 6 from top to bottom of the plant and were cured in different curing barns (T1 as the control barn cured 6 leaves from six positions; T2 includes 2 barns, one barn with position 1, 2 and 3 leaves, the other with position 4, 5, 6 leaves; T3 includes 3 barns, one barn with position 1 and 2 leaves, one barn with position 3 and 4 leaves and the other barn with position 5 and 6 leaves). Single leaf weigh, stem ratio, appearance quality and grade structure of the cured upper leaves were investigated and chemical component coordination and sensory quality of the upper cured leaves were evaluated. The main results are given as follows: ① for leaves in T3 barns, single leaf weigh of position 5 and 6 leaves were increased by 9.4% and 11.2%, respectively, and the stem ratios in position 1 to leaf 6 leaves were reduced by 5.2%,3.3%,2.5%,6.9%,8.7% and 13.1%,respectively, compared to leaves in control barn; ② compared to leaves in control barn, the percentages of dark golden yellow, mature, loose, oily and chroma intensity leaf in cured leaves from T1 and T2 barns were significantly higher; ③ Curing in different curing barns by leaf position could significantly increase the proportion of superior leaf of the cured upper leaves. For position 1 to 3 leaves in T3 barns, the proportion of B1F and B2F grade leaves were increased by 8.54 and the proporting of B2F was increased by 48.4%, whereas the proportion of B1F and B2F grade among leaf position four to position six were increased by 195.6% and 10.5%, respectively; ④ Reducing sugar content, ratio of reducing sugar content to total sugar and sugar base ratio in leaves cured in T2 and T3 barns were significantly increased, compared to leaves cured in control barn (P＜0.05); ⑤ the sensory evaluation scores of aroma quantity, soft-delicate, thrill, aftertaste, miscellaneous gas and total sensory score of the cured upper leaves under curing in different curing barns by leaf position were higher than those of curing in the same curing barn.