In order to screen suitable moss mats and substrates for Leucobryum glaucum breeding, and to improve the artificial reproduction rate and quality of Leucobryum glaucum, the Leucobryum glaucum stems was cultured on 5 kinds of moss mats(moss fabric, felt, non-woven fabrics, sponge, 3D moss fabric) and 6 kinds of substrates(ceramsite, kanuma mud, vermiculite, Zhijing stone, red clay, perlite) for 120 days. The biomass, color score, chlorophyll content and coverage of Leucobryum glaucum were detected. The results showed that on different moss mats, the biomass, color score, chlorophyll content and coverage on moss fabric were 2064 g/m2, 4.33, 2.16 mg/L and 50.33%, respectively; while on different substrates, the biomass, color score, chlorophyll content and coverage on red clay were 9072 g/m2, 8, 3.15 mg/L, 83.31%, respectively. The suitable moss mats and substrates for the growth of Leucobryum glaucum are moss fabric and red clay.