150 SPF chicks were randomly grouped into 5 groups, control group(CK), virus infection model group(MK), traditional Chinese medicine extract high, medium and low dose group(T1, T2, T3). The T1, T2 and T3 group’ chicks were respectively treated by the extract mixed with drinking water at the doses of 10.0, 5.0, 2.5 g/L for 7 consecutive days. The extracts included LIGUSTRI LUCIDI FRUCTUS, ASTRAGALI RADIX, LYCII FRUCTUS, CUSCUTAE SEMEN with the mass ratio of 2∶2∶1∶1. The CK and MC group were added without any drugs. Then, the chicks in the CK group were nasal saline as control, the others were inoculated with IBDV via nasal drops with observation for 7 continuous days. Detected the antioxidant indexes(T-SOD, GSH-Px, T-AOC, MDA) and inflammatory factors(NO, TNF-ɑ, IL-1β, IL-6), and tested iNOS, NF-κB and MCP-1 mRNA expression in the spleen lymph cell. The results showed that the chicks in T1 and T2 group had increased their antioxidant capacity, serum GSH-Px and T-AOC activity were extremely significantly higher than those in MC group(P<0.01), serum MDA content was significant lower than that in MC group(P<0.05). The T-SOD activity in T2 group was significantly higher than that in MC group(P<0.05). Meanwhile, the chicks in T1 and T2 group had reduced their inflammatory response, NO, TNF-α and IL-1β content were extremely significantly lower than those in MC group(P<0.01), of which serum NO and TNF-ɑ contents were significantly lower than those of T3 group(P<0.05), IL-1β content was significantly or extremely significantly lower than that in T3 group, and IL-6 content in T2 group was significantly higher than that in T3 and MC group(P<0.05). And, the expression of iNOS mRNA in spleen lymphocytes of chicks in T1 and T2 groups had been significantly decreased than that in MC(P<0.05), and the expression of NF-κB and MCP-1 mRNA had been extremely significantly decreased than that in MC(P<0.01). These results indicated that the traditional Chinese medicine extract might resist IBDV by enhancing the anti-oxidation ability of chickens, promoting the secretion of inflammatory related factors and regulating the expression of genes to achieve anti-inflammatory effects.